Western blot analysis of lysates from mouse liver, heart tissue and A431 cell line (from left to right), using ATP5I Antibody (C-term)(Cat. #AW5003). AW5003 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.
Immunohistochemical analysis of paraffin-embedded H. liver section using ATP5I Antibody (C-term)(Cat#AW5003). AW5003 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Immunohistochemical analysis of paraffin-embedded M. heart section using ATP5I Antibody (C-term)(Cat#AW5003). AW5003 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Immunohistochemical analysis of paraffin-embedded H. kidney section using ATP5I Antibody (C-term)(Cat#AW5003). AW5003 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Fluorescent image of Hela cells stained with ATP5I Antibody (C-term)(Cat#AW5003). AW5003 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
Fluorescent image of Hela cells stained with ATP5I Antibody (C-term)(Cat#AW5003). AW5003 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).