All lanes : Anti-Aftiphilin antibody - C-terminal (ab175484) at 1/1000 dilutionLane 1 : CEM cell line lysateLane 2 : Jurkat cell line lysateLane 3 : MCF7 cell line lysateLysates/proteins at 35 µg per lane.
Immunohistochemical analysis of formalin fixed, paraffin embedded Human brain tissue labeling Aftiphilin with ab175484 at 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of HL60 cells labeling Aftiphilin with ab175484 antibody at 1/10 dilution (right histogram), compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.