Western blot analysis of lysates from Hela cell line,mouse brain,mouse cerebellum tissue,mouse NIH/3T3,rat PC-12 cell line,rat brain,rat cerebellum tissue,U-251 MG cell line (from left to right), using PITPNA Antibody (C-term)(Cat. #AW5110). AW5110 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.
Immunohistochemical analysis of paraffin-embedded H. skin section using PITPNA Antibody (C-term)(Cat#AW5110). AW5110 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
All lanes : Anti-PITPNA Antibody (C-term) at 1:2000 dilution Lane 1: NIH/3T3 whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: Hela whole cell lysate Lane 4: MOLT-4 whole cell lysate Lane 5: U-251 MG whole cell lysate Lane 6: mouse brain lysate Lane 7: rat brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 32 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes : Anti-PITPNA Antibody (C-term) at 1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: MOLT-4 whole cell lysate Lane 3: Hela whole cell lysate Lane 4: U-251 MG whole cell lysate Lane 5: mouse brain lysate Lane 6: NIH/3T3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 32 kDa Blocking/Dilution buffer: 5% NFDM/TBST.