Western blot analysis of lysates from Hela,HL-60,SW480 cell line (from left to right), using NDUA4 Antibody (C-term)(Cat. #AW5303). AW5303 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
Fluorescent image of Hela cells stained with NDUA4 Antibody (C-term)(Cat#AW5303). AW5303 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
Immunohistochemical analysis of paraffin-embedded H. kidney section using NDUA4 Antibody (C-term)(Cat#AW5303). AW5303 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.