Anti-AGE antibody
| Name | Anti-AGE antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab23722 |
| Prices | $400.00 |
| Sizes | 200 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF ELISA IHC-F IHC-P |
| Antigen | Advanced Glycation End Products (BSA-AGE and HSA-AGE) |
| Blocking Peptide | AGE-BSA full length protein |
| Description | Rabbit Polyclonal |
| Gene | RENBP |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-AGE antibody (ab23722) at 1/1000 dilutionLane 1 : Mouse Brain Lysate(Brain and Hippocampus) at 25 µgLane 2 : Glycated BSA at 15 µgSecondaryHRP-conjugated Goat anti-rabbit IgG at 1/800 dilutionPerformed under reducing conditions.This image is courtesy of an Abreview submitted by Josephine Böhme12% SDS-PAGE gel run using denaturing conditions. Membrane blocked with 5% BSA for 30 minutes at room temperature. Primary antibody diluted in 5% BSA in TBS-T and incubated for 20 hours in a fridgeSee Abreview
ab23722 (1µg/ml) staining AGE in human colon using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of both cell membrane and cytoplasm.Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
ICC/IF image of ab23722 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab23722, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
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