Western blot analysis of lysates from A431,KG-1 cell line,mouse kidney,mouse liver tissue lysate,SH-SY5Y cell line (from left to right), using CLN3 Antibody (Center)(Cat. #AW5327). AW5327 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.
Flow cytometric analysis of Hela cells using CLN3 Antibody (Center)(green, Cat#AW5327c) compared to an isotype control of rabbit IgG(blue). AW5327 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
Immunohistochemical analysis of paraffin-embedded H. liver section using CLN3 Antibody (Center)(Cat#AW5327). AW5327 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.