All lanes : Anti-SLC25A37 Antibody (C-term) at 1:1000 dilution Lane 1: K562 whole cell lysates Lane 2: rat spleen lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 37 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
SLC25A37 Antibody (C-term) (Cat. #AW5376) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SLC25A37 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
SLC25A37 Antibody (C-term) (Cat. #AW5376) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Immunofluorescent analysis of Hela cells, using SLC25A37 Antibody (C-term) (Cat. #AW5376). AW5376 was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor® 554 (red) conjugated Phalloidin (red).