Anti-Aggrecan antibody
| Name | Anti-Aggrecan antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab36861 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P WB ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human, Dog |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Rat Aggrecan |
| Description | Rabbit Polyclonal |
| Gene | ACAN |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
IHC image of Aggrecan staining in rat liver formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab36861, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Anti-Aggrecan antibody (ab36861) at 1 µg/ml + Aggrecan G1-IGD-G2 recombinant protein SecondaryGoat polyclonal to Rabbit IgG H&L (HRP) at 1/10000 dilutionPerformed under reducing conditions.
IHC image of Aggrecan staining in human liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab36861, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
All lanes : Anti-Aggrecan antibody (ab36861) at 1 µg/mlLane 1 : Liver (Mouse) Tissue LysateLane 2 : Liver (Rat) Tissue LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Product References
Association of endothelin-1 expression and cartilaginous endplate degeneration in - Association of endothelin-1 expression and cartilaginous endplate degeneration in
Yuan W, Zhao MD, Yuan FL, Che W, Duan PG, Liu Y, Dong J. PLoS One. 2013;8(4):e60062.
RB1CC1 protein suppresses type II collagen synthesis in chondrocytes and causes - RB1CC1 protein suppresses type II collagen synthesis in chondrocytes and causes
Nishimura I, Chano T, Kita H, Matsusue Y, Okabe H. J Biol Chem. 2011 Dec 23;286(51):43925-32.
In vitro chondrogenic differentiation of human mesenchymal stem cells in collagen - In vitro chondrogenic differentiation of human mesenchymal stem cells in collagen
Hui TY, Cheung KM, Cheung WL, Chan D, Chan BP. Biomaterials. 2008 Aug;29(22):3201-12.