Anti-AGR3 antibody [AGR3.1]
| Name | Anti-AGR3 antibody [AGR3.1] |
|---|---|
| Supplier | Abcam |
| Catalog | ab82400 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | AGR3.1 |
| Applications | WB IHC-P IHC-F ICC/IF |
| Species Reactivities | Human |
| Antigen | Purified human AGR3 protein |
| Description | Mouse Monoclonal |
| Gene | AGR3 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunohistochemistry staining of AGR3 with ab82400 at 1ug/ml in paraffin-embedded sections of breast carcinoma.
![All lanes : Anti-AGR3 antibody [AGR3.1] (ab82400) at 1 µg/mlLane 1 : H1229 lung carcinoma cell lineLane 2 : T47D breast cancer cell line](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/3658_AGR3-Primary-antibodies-ab82400-3.JPG)
All lanes : Anti-AGR3 antibody [AGR3.1] (ab82400) at 1 µg/mlLane 1 : H1229 lung carcinoma cell lineLane 2 : T47D breast cancer cell line
ICC/IF image of ab82400 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab82400, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.