Human primary fibroblasts stained with ab68556 (green). Actin filaments were decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).
ab68556 at 1/250 dilution staining AHNAK (red) in human Hela cells by Immunocytochemistry/Immunofluorescence. Nuclei were stained with DAPI (blue).
![Anti-AHNAK antibody [EM-09] (ab68556) at 2 µg/mlPerformed under non-reducing conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/3757_AHNAK-Primary-antibodies-ab68556-1.jpg)
Anti-AHNAK antibody [EM-09] (ab68556) at 2 µg/mlPerformed under non-reducing conditions.
Ab68556 (red) staining AHNAK in murine tongue by immunohistochemistry using frozen tissue sections. Actin filaments were decorated by phalloidin (green), cell nuclei stained with DAPI (blue).
![Overlay histogram showing HeLa cells stained with ab68556 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab68556, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Hela cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/3759_AHNAK-Primary-antibodies-ab68556-5.jpg)
Overlay histogram showing HeLa cells stained with ab68556 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab68556, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Hela cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.