Anti-AIRE antibody
| Name | Anti-AIRE antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab82493 |
| Prices | $376.00 |
| Sizes | 200 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P WB |
| Species Reactivities | Human, Mouse |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human AIRE |
| Description | Rabbit Polyclonal |
| Gene | AIRE |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-AIRE antibody (ab82493) at 1/250 dilution + Thymus (Human) Tissue Lysate - adult normal tissue (ab30146) at 10 µgSecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
IHC image of AIRE staining in human testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with 82493, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX