All lanes : Anti-AKAP14 antibody [EPR12955] - C-terminal (ab180160) at 1/1000 dilutionLane 1 : Jurkat cell lysateLane 2 : HUVEC cell lysateLane 3 : NCI-H460 cell lysateLane 4 : A549 cell lysateLane 5 : Human fetal kidney lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilutiondeveloped using the ECL technique
Immunofluorescence analysis of A549 cells labeling AKAP14 with ab180160 at a 1/100 dilution.
Western blot analysis on Immunoprecipitation pellet from either 1) Jurkat cell lysate, or 2) 1xPBS (negative control); showing AKAP14, using ab180160 at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.