Flow cytometric analysis of permeabilized Jurkat cells labeling AKAP3 with ab170856 at 1/100 dilution (red) or a rabbit IgG (negative) (green).
Immunofluorescent analysis of HeLa cells labeling AKAP3 with ab170856 at 1/250 dilution (red). DAPI nuclear staining (blue).
All lanes : Anti-AKAP3 antibody [EPR11261] (ab170856) at 1/1000 dilutionLane 1 : Y79 cell lysateLane 2 : HuT-78 cell lysateLane 3 : HeLa cell lysateLane 4 : 293T cell lysateLane 5 : NIH 3T3 cell lysateLane 6 : NCI-H460 cell lysateLane 7 : Jurkat cell lysateLysates/proteins at 10 µg per lane.