Anti-AKT1 (phospho S473) antibody
| Name | Anti-AKT1 (phospho S473) antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab66138 |
| Prices | $403.00 |
| Sizes | 200 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P ICC/IF ICC/IF ELISA DB IHC-F WB IP |
| Species Reactivities | Mouse, Rat, Chicken, Dog, Human |
| Antigen | Synthetic peptide within Human AKT1 (C terminal) (phospho S473) |
| Blocking Peptide | AKT1 peptide |
| Description | Rabbit Polyclonal |
| Gene | AKT1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-AKT1 (phospho S473) antibody (ab66138) at 1/1000 dilutionLane 1 : Whole cell lysate derived from PDGF stimulated 3T3Lane 2 : Whole cell lysate derived from PDGF stimulated 3T3 with immunising peptideLysates/proteins at 10 µg per lane.
ab66138 staining AKT1 in Mouse embryonic stem cells by Immunocytochemistry/Immunofluorescence. The cells were 4% PFA fixed and permeabilized in 0.1% Triton prior to blocking in 10% serum for 30 minutes at room temperature. The primary antibody was diluted 1/1000 and incubated with the sample for 12 hours at 4°C. The secondary antibody was an Alexa Fluor® 594-conjugated Goat anti-Rabbit polyclonal (ab150160), diluted 1/1500.
ICC/IF image of ab66138 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab66138 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab66138 staining phospho AKT1 in Mouse embryonic tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Triton and blocked with 10% serum for 1 hour at 22°C. Samples were incubated with primary antibody (1/500 in blocking buffer (1:10)) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Goat anti-rabbit polyclonal (1/1000)ab150077) was used as the secondary antibody.See Abreview
developed using the ECL techniquePerformed under reducing conditions.
1µg peptide blotted onto a nitrocellulose membrane, followed by ab66138 at 1:2000 dilution. A: AKT1 (pS473) peptide B: AKT1 non-phosphopeptide C: non-related phosphopeptide
Product References
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