Anti-Alas1 antibody - Mitochondrial Marker
| Name | Anti-Alas1 antibody - Mitochondrial Marker |
|---|---|
| Supplier | Abcam |
| Catalog | ab101681 |
| Prices | $376.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF |
| Species Reactivities | Human, Mouse, Rat, Horse, Bovine, Monkey |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human Alas1 |
| Description | Rabbit Polyclonal |
| Gene | ALAS1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab101681 stained T47D cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab101681, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab101681, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Anti-Alas1 antibody - Mitochondrial Marker (ab101681) at 1 µg/ml + HepG2 (Human hepatocellular liver carcinoma cell line) Nuclear Lysate at 10 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.