Anti-Albumin antibody - C-terminal (ab135575) at 1/1000 dilution + Mouse liver tissue lysate at 35 µgSecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/5000 dilution
Anti-Albumin antibody - C-terminal (ab135575) at 1/1000 dilution + 293 cell lysate at 35 µgSecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/5000 dilution
Flow cytometric analysis of HepG2 cells labelling Alubmin with ab135575 (green) compared to a negative control cell (blue). Cells were fixed with paraformaldehyde and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 3% BSA to block non-specific protein-protein interactions followed by the antibody (ab135575, 1µg/1x10^6 cells) for 60 min at 37ºC. The secondary antibody used was a FITC-conjugated goat anti-rabbit IgG at 1/200 dilution for 40 min at room temperature. Blank control (blue line) was used under the same conditions.Acquisition of >10,000 events was performed.