![ALDH1A2 was immunoprecipitated using 0.5mg Mouse Kidney tissue extract, 5µg of Rabbit polyclonal to ALDH1A2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, Mouse Kidney tissue extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab96060.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 57kDa; ALDH1A2](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/4689_ab96060-209869-IPVI004ab9606020mMod.jpg)
ALDH1A2 was immunoprecipitated using 0.5mg Mouse Kidney tissue extract, 5µg of Rabbit polyclonal to ALDH1A2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, Mouse Kidney tissue extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab96060.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 57kDa; ALDH1A2
Anti-ALDH1A2 antibody (ab96060) at 1/1000 dilution + IMR32 whole cell lysate at 30 µg
All lanes : Anti-ALDH1A2 antibody (ab96060) at 1/1000 dilutionLane 1 : Mouse Liver extractLane 2 : Mouse Kidney extractLysates/proteins at 100 µg per lane.SecondaryHRP conjugated Goat anti-rabbit at 1/4000 dilution
Anti-ALDH1A2 antibody (ab96060) + HeLa cell lysate at 100 µgSecondaryHRP-conjugated goat anti-rabbit polyclonal IgG at 1/4000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ab96060 staining ALDH1A2 in Human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% serum for 20 minutes at 22°C; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/200 in 1% BSA in PBS buffer) for 16 hours at 4°C. An Alexa Fluor®594-conjugated Goat anti-rabbit IgG polyclonal(1/200) was used as the secondary antibody.See Abreview
ab96060 staining ALDH1A2 in Mouse colon tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde and blocked with 5% serum for 20 minutes at 25°C. Samples were incubated with primary antibody (1/200 in PBS + 1% BSA) for 16 hours at 4°C. An AlexaFluor®594-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.See Abreview