Detection of Human ALG2 by Western Blot of Immunoprecipitate. HepG2 cell lysate (lane 1) and a negative control (lane 2) loaded, ab181124 used to immunoprecipitate ALG2 at a 1/50 dilution. Anti-Rabbit IgG (HRP) specific to the non-reduced form of IgG secondary used at a 1/1500 dilution.
Immunofluorescence analysis of 293 cells (fixative 4% paraformaldehyde) labeling ALG2 with ab181124 at a 1/250 dilution.Goat anti rabbit IgG (Alexafluor 555) secondary used at a 1/200 diution.
Immunohistochemical analysis of paraffin embedded Human testis tissue labeling ALG2 with ab181124 at a 1/100 dilution. Prediluted HRP Polymer for Rabbit IgG secondary used. Counterstained with Hematoxylin.
Immunohistochemical analysis of paraffin embedded Human pancreas tissue labeling ALG2 with ab181124 at a 1/100 dilution. Prediluted HRP Polymer for Rabbit IgG secondary used. Counterstained with Hematoxylin.
All lanes : Anti-ALG2 antibody [EPR12007(2)(B)] (ab181124) at 1/5000 dilutionLane 1 : HepG2 lysateLane 2 : A431 lysateLane 3 : 293 lysateLane 4 : SK-OV-3 lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/5000 dilution