Anti-Alkaline Phosphatase antibody [EPR15201(B)] (ab186422) at 1/10000 dilution + HeLa cell lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
All lanes : Anti-Alkaline Phosphatase antibody [EPR15201(B)] (ab186422) at 1/1000 dilutionLane 1 : JAR cell lysateLane 2 : Human placenta lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human small intestine tissue labeling Alkaline Phosphatase with ab186422 at 1/500 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.