Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human testis tissue, labeling ALKBH6 with ab170186 at 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.
Anti-ALKBH6 antibody (ab170186) at 1/100 dilution + Mouse cerebellum tissue lysate at 35 µg
Anti-ALKBH6 antibody (ab170186) at 1/100 dilution + MDA-MB435 cell line lysate at 35 µg
Flow cytometric analysis of MDA-MB435 cells, labeling ALKBH6 with ab170186 at 1/10 dilution (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.