Western blot analysis of immunoprecipitation pellet from Raji cell lysate immunoprecipitated using ab192614 at 1/50 dilution (lane 1) or PBS control (lane 2).Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
All lanes : Anti-alpha 1 Adrenergic Receptor antibody [EPR11821(2)] (ab192614) at 1/1000 dilutionLane 1 : RAW 264.7 cell lysateLane 2 : NIH 3T3 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
All lanes : Anti-alpha 1 Adrenergic Receptor antibody [EPR11821(2)] (ab192614) at 1/1000 dilutionLane 1 : Raji cell lysateLane 2 : Human fetal heart lysateLysates/proteins at 20 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution