ab46799 staining alpha 1 Fetoprotein in cat liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 2.5% serum for 30 minutes at 25°C; antigen retrieval was by heat mediation in 10mM citrate buffer, pH 6.0. Samples were incubated with primary antibody (1/50 in PBS + 1X casein) for 1 hour at 25°C. An undiluted HRP-conjugated horse anti-rabbit IgG polyclonal was used as the secondary antibody.See Abreview
Anti-alpha 1 Fetoprotein antibody (ab46799) at 1/50000 dilution + HepG2 cell lysate at 10 µgSecondaryGoat anti-rabbit HRP labeled, at 1/2000 dilution
ab46799 staining alpha 1 Fetoprotein in Mouse embryonic liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formalin and blocked with 2.5% serum for 20 minutes at 23°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/750 in 1% BSA in TBS) for 1 hour at 23°C. An undiluted HRP-conjugated Horse anti-rabbit polyclonal was used as the secondary antibody.See Abreview