Anti-alpha 2 Glycine Receptor antibody (ab97628) at 1/5000 dilution + A431 whole cell lysate at 30 µg
ab97628, at a 1/500 dilution, staining alpha 2 Glycine Receptor in paraffin embedded MDAMB468 xenograft by Immunohistochemistry.
ICC/IF image of ab97628 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab97628 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.