Anti-alpha Adducin antibody
| Name | Anti-alpha Adducin antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab51130 |
| Prices | $387.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P WB ELISA ICC/IF ICC/IF IP |
| Species Reactivities | Human, Mouse, Rat |
| Antigen | Synthesized non-phosphopeptide derived from human alpha Adducin around the phosphorylation site of serine 726 (T-P-SP-F-L) |
| Description | Rabbit Polyclonal |
| Gene | ADD1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
This image shows paraffin-embedded human brain tissue stained with ab51130 at a dilution of 1/100. Right hand image: tissue treated with immunising peptide; left hand image: untreated tissue
All lanes : Anti-alpha Adducin antibody (ab51130)Lane 1 : Extracts from Hela cells treated with Forskolin (40nM, 30min) with no peptideLane 2 : Extracts from Hela cells treated with Forskolin (40nM, 30min) with with immunising peptide
ICC/IF image of ab51130 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51130, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab51130 used in Immunoprecipitation and Western Blot.Immunoprecipitation of NLN (EC 3.4.24.16), Thimet Oligopeptidase (thimet OP; EC 3.4.24.15), a-Adducin, and collapsin response mediator protein 2 (CRMP2) from photoradiolabeled P10 mouse forebrain membrane preparations. Immunoprecipitation of each protein was carried out from parallel nonspecific (NS) and total (Tot) photoradiolabeled groups.a, equal amounts of NS and total sample of each protein immunoprecipitate were used to count the radioactive signal. Bck, background radioactivity; ***, p < 0.001 versus all other groups; n = 3–4).b–e, representative Western blot confirmation of successful immunoprecipitation of each candidate protein from NS and total samples.
Product References
Axon initial segment cytoskeleton comprises a multiprotein submembranous coat - Axon initial segment cytoskeleton comprises a multiprotein submembranous coat
Jones SL, Korobova F, Svitkina T. J Cell Biol. 2014 Apr 14;205(1):67-81.
Identification of membrane-bound variant of metalloendopeptidase neurolysin (EC - Identification of membrane-bound variant of metalloendopeptidase neurolysin (EC
Wangler NJ, Santos KL, Schadock I, Hagen FK, Escher E, Bader M, Speth RC, Karamyan VT. J Biol Chem. 2012 Jan 2;287(1):114-22.