Anti-alpha Glucosidase II antibody (ab96757) at 1/1000 dilution + NIH-3T3 whole cell lysate at 30 µg
All lanes : Anti-alpha Glucosidase II antibody (ab96757) at 1/1000 dilutionLane 1 : 293T whole cell lysateLane 2 : A431 whole cell lysateLane 3 : H1299 whole cell lysateLane 4 : HeLa S3 whole cell lysateLane 5 : HepG2 whole cell lysateLane 6 : Molt-4 whole cell lysateLane 7 : Raji whole cell lysateLysates/proteins at 30 µg per lane.
Immunohistochemical analysis of alpha Glucosidase II in paraffin-embedded SW480 xenograft, using ab96757 at 1/500 dilution.
Immunofluorescence analysis of alpha Glucosidase II in methanol-fixed HeLa cells, using ab96757 at 1/200 dilution (1) and costained with Hoechst 33342 (2).