Anti-alpha Internexin antibody
| Name | Anti-alpha Internexin antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab7259 |
| Prices | $390.00 |
| Sizes | 50 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-F IHC-P IHC-F ICC/IF ICC/IF |
| Species Reactivities | Rat, Human, Mammalian |
| Antigen | Recombinant full length protein (Rat) |
| Description | Rabbit Polyclonal |
| Gene | INA |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Ab7259 shows red, neuronal progenitor cells, plectin (not one of our antibodies) shows fibroblast marker. Photo courtesy of: Dr. Gerry Shaw University of Florida
Anti-alpha Internexin antibody (ab7259) at 1/20000 dilution + whole rat spinal cord homogenate
Ab7259 staining Human normal temporal cortex. Staining is localised to intracellualr compartment.Left panel: with primary antibody diluted 1:2000. Right panel: isotype control.Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
ICC/IF image of ab7259 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7259, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Encoding and transducing the synaptic or extrasynaptic origin of NMDA receptor - Encoding and transducing the synaptic or extrasynaptic origin of NMDA receptor
Karpova A, Mikhaylova M, Bera S, Bar J, Reddy PP, Behnisch T, Rankovic V, Spilker C, Bethge P, Sahin J, Kaushik R, Zuschratter W, Kahne T, Naumann M, Gundelfinger ED, Kreutz MR. Cell. 2013 Feb 28;152(5):1119-33.
Proteomics reveal rat hippocampal lateral asymmetry. - Proteomics reveal rat hippocampal lateral asymmetry.
Samara A, Vougas K, Papadopoulou A, Anastasiadou E, Baloyanni N, Paronis E, Chrousos GP, Tsangaris GT. Hippocampus. 2011 Jan;21(1):108-19.
The clinical diagnosis of early-onset dementias: diagnostic accuracy and - The clinical diagnosis of early-onset dementias: diagnostic accuracy and
Snowden JS, Thompson JC, Stopford CL, Richardson AM, Gerhard A, Neary D, Mann DM. Brain. 2011 Sep;134(Pt 9):2478-92.