Anti-alpha Internexin antibody [2E3]
| Name | Anti-alpha Internexin antibody [2E3] |
|---|---|
| Supplier | Abcam |
| Catalog | ab7654 |
| Prices | $390.00 |
| Sizes | 250 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 2E3 |
| Applications | IHC-F WB FC ICC/IF ICC/IF IHC-P |
| Species Reactivities | Rat, Human, Mammalian |
| Antigen | Recombinant full length protein (Rat) |
| Description | Mouse Monoclonal |
| Gene | INA |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Hippocampal neurons in tissue culture with neurofilament alpha internexin (red) (Ab7654) and GFAP (green). Photo courtesy of: Dr. Gerry Shaw University of Florida
![All lanes : Anti-alpha Internexin antibody [2E3] (ab7654)Lane 1 : Crude homogenate of rat facial nucleus at 3 days after experimental injuryLane 2 : Crude homogenate of rat facial nucleus at 3 days after experimental injuryLane 3 : Crude homogenate of rat facial nucleus at 5 days after experimental injuryLane 4 : Crude homogenate of rat facial nucleus at 5 days after experimental injuryLane 5 : Crude homogenate of rat facial nucleus at 7 days after experimental injuryLane 6 : Crude homogenate of rat facial nucleus at 7 days after experimental injuryLane 7 : Crude homogenate of rat facial nucleus at 14 days after experimental injuryLane 8 : Crude homogenate of rat facial nucleus at 14 days after experimental injuryLane 9 : Crude homogenate of rat facial nucleus at 28 days after experimental injuryLane 10 : Crude homogenate of rat facial nucleus at 28 days after experimental injury](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/5783_ab7654_2.jpg)
All lanes : Anti-alpha Internexin antibody [2E3] (ab7654)Lane 1 : Crude homogenate of rat facial nucleus at 3 days after experimental injuryLane 2 : Crude homogenate of rat facial nucleus at 3 days after experimental injuryLane 3 : Crude homogenate of rat facial nucleus at 5 days after experimental injuryLane 4 : Crude homogenate of rat facial nucleus at 5 days after experimental injuryLane 5 : Crude homogenate of rat facial nucleus at 7 days after experimental injuryLane 6 : Crude homogenate of rat facial nucleus at 7 days after experimental injuryLane 7 : Crude homogenate of rat facial nucleus at 14 days after experimental injuryLane 8 : Crude homogenate of rat facial nucleus at 14 days after experimental injuryLane 9 : Crude homogenate of rat facial nucleus at 28 days after experimental injuryLane 10 : Crude homogenate of rat facial nucleus at 28 days after experimental injury
![Overlay histogram showing SH-SY5Y cells stained with ab7654 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab7654, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/5784_alpha-Internexin-Primary-antibodies-ab7654-11.jpg)
Overlay histogram showing SH-SY5Y cells stained with ab7654 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab7654, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
ab7654 staining alpha Internexin in human cerebral cortex tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% hydrogen peroxide for 10 minutes at 25°C; antigen retrieval was by heat mediation in EDTA, pH 9.0 (100°C for 20 minutes). Samples were incubated with primary antibody (1/2500) for 20 minutes at 25°C.See Abreview
Product References
Expression of intermediate filament proteins and neuronal markers in the human - Expression of intermediate filament proteins and neuronal markers in the human
Rauch U, Klotz M, Maas-Omlor S, Wink E, Hansgen A, Hagl C, Holland-Cunz S, Schafer KH. J Histochem Cytochem. 2006 Jan;54(1):39-46. Epub 2005 Aug 8.
Screening for neurofilament inclusion disease using alpha-internexin - Screening for neurofilament inclusion disease using alpha-internexin
Uchikado H, Shaw G, Wang DS, Dickson DW. Neurology. 2005 May 10;64(9):1658-9.
The expression of alpha-internexin and peripherin in the developing mouse pineal - The expression of alpha-internexin and peripherin in the developing mouse pineal
Ko TL, Chien CL, Lu KS. J Biomed Sci. 2005 Oct;12(5):777-89. Epub 2005 Nov 9.