![Anti-alpha Internexin antibody [EP676Y] (ab40758) at 1/10000 dilution + SHSY5Y cell lysate at 10 µg](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/5786_40758a.jpg)
Anti-alpha Internexin antibody [EP676Y] (ab40758) at 1/10000 dilution + SHSY5Y cell lysate at 10 µg
ab40758 staining alpha Internexin in rat brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with formaldehyde, permeablized with 0.01% Triton X-100, blocked with 5% serum for 2 hours at 4°C and antigen retrieval was by heat mediation in citrate buffer. The sample was incubated with primary antibody (1/250) at 4°C for 10 hours. An undiluted Alexa Fluor® 555-conjugated anti-rabbit IgG polyclonal was used as the secondary antibody.See Abreview
Positive staining of alpha Internexin on paraffin-embedded human brain tissue (A) using ab40758 diluited 1:250. Negative staining of paraffin-embedded human colon (B), Kidney (C) and uterus (D) tissues using ab40758 under the same conditions.
ab40758 staining alpha Internexin in rat glial cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with saponin and blocked with 1% BSA for 10 minutes at 25°C. Samples were incubated with primary antibody (1/200) for 10 hours at 4°C. An undiluted Alexa Fluor® 594-conjugated donkey anti-rabbit IgG polyclonal was used as the secondary antibody.See Abreview
Immunofluorescent staining of alpha Internexin on PC 12 cells using ab40758 diluted 1:250.