Anti-alpha Tubulin antibody [B-5-1-2]

• Supplier: Abcam
• Catalog: ab11304
• Prices: $400.00
• Sizes: 100 µl
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: B-5-1-2
• Applications: WB RIA IP ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Chicken, Bovine, Human, Chlamydomonas reinhardtii, Sea Urchin, Dog, Pig, Xenopus, Monkey, Monkey, Xenopus
• Antigen: Full length protein corresponding to alpha Tubulin
• Description: Mouse Monoclonal
• Gene: TUBA4A
• Conjugate: Unconjugated

Product images

All lanes : Anti-alpha Tubulin antibody [B-5-1-2] (ab11304) at 1/2000 dilutionLane 1 : Whole cell lysate prepared from HEK293 cellsLane 2 : Whole cell lysate prepared from HEK293 cellsLane 3 : Whole cell lysate prepared from HEK293 cellsLane 4 : Whole cell lysate prepared from HEK293 cellsLane 5 : Whole cell lysate prepared from HEK293 cellsLane 6 : Whole cell lysate prepared from HEK293 cellsLysates/proteins at 30 µg per lane.SecondaryHRP conjugated mouse anti-rabbit at 1/5000 dilutiondeveloped using the ECL technique

ab11304 staining  alpha Tubulin in the HEK293T cell line by ICC/IF (Immunocytochemistry/immunofluorescence) transfected with a recombinant protein HA tagged dual stained with Anti-HA Mouse primary antibody and Anti-Mouse Alexa Fluor®543 secondary antibody. Microtubules detected with Anti-α-tubulin Mouse primary antibody and Anti-Mouse Alexa Fluor®488 secondary antibody.Nuclus visualised with DAPI.

ab11304 at a 1/100 dilution staining human HeLa cells by Immunocytochemistry/Immunofluorescence. The cells were methanol fixed and blocked for 1 hour with 10% serum. The antibody was then incubated with the cells for 1 hour. Bound antibody was detected using a Goat anti-mouse TRITC antibody. See Abreview

Product References

MicroRNA-146b promotes myogenic differentiation and modulates multiple gene - MicroRNA-146b promotes myogenic differentiation and modulates multiple gene

Khanna N, Ge Y, Chen J. PLoS One. 2014 Jun 23;9(6):e100657.

Premature termination of SMARCB1 translation may be followed by reinitiation in - Premature termination of SMARCB1 translation may be followed by reinitiation in

Hulsebos TJ, Kenter S, Verhagen WI, Baas F, Flucke U, Wesseling P. Acta Neuropathol. 2014 Sep;128(3):439-48.

Similar skeletal muscle angiogenic and mitochondrial signalling following 8 weeks - Similar skeletal muscle angiogenic and mitochondrial signalling following 8 weeks

Malek MH, Huttemann M, Lee I, Coburn JW. Exp Physiol. 2013 Mar;98(3):807-18.

Interferon beta induces clearance of mutant ataxin 7 and improves locomotion in - Interferon beta induces clearance of mutant ataxin 7 and improves locomotion in

Chort A, Alves S, Marinello M, Dufresnois B, Dornbierer JG, Tesson C, Latouche M, Baker DP, Barkats M, El Hachimi KH, Ruberg M, Janer A, Stevanin G, Brice A, Sittler A. Brain. 2013 Jun;136(Pt 6):1732-45.

Genomic and proteomic analyses of Prdm5 reveal interactions with insulator - Genomic and proteomic analyses of Prdm5 reveal interactions with insulator

Galli GG, Carrara M, Francavilla C, de Lichtenberg KH, Olsen JV, Calogero RA, Lund AH. Mol Cell Biol. 2013 Nov;33(22):4504-16.

Mammalian target of rapamycin and Rictor control neutrophil chemotaxis by - Mammalian target of rapamycin and Rictor control neutrophil chemotaxis by

He Y, Li D, Cook SL, Yoon MS, Kapoor A, Rao CV, Kenis PJ, Chen J, Wang F. Mol Biol Cell. 2013 Nov;24(21):3369-80.

Interplay between kinase domain autophosphorylation and F-actin binding domain in - Interplay between kinase domain autophosphorylation and F-actin binding domain in

Preyer M, Vigneri P, Wang JY. PLoS One. 2011 Feb 11;6(2):e17020.

miR-449 inhibits cell proliferation and is down-regulated in gastric cancer. - miR-449 inhibits cell proliferation and is down-regulated in gastric cancer.

Bou Kheir T, Futoma-Kazmierczak E, Jacobsen A, Krogh A, Bardram L, Hother C, Gronbaek K, Federspiel B, Lund AH, Friis-Hansen L. Mol Cancer. 2011 Mar 18;10:29.

MicroRNA-145 targets YES and STAT1 in colon cancer cells. - MicroRNA-145 targets YES and STAT1 in colon cancer cells.

Gregersen LH, Jacobsen AB, Frankel LB, Wen J, Krogh A, Lund AH. PLoS One. 2010 Jan 21;5(1):e8836.

Mammalian target of rapamycin regulates miRNA-1 and follistatin in skeletal - Mammalian target of rapamycin regulates miRNA-1 and follistatin in skeletal

Sun Y, Ge Y, Drnevich J, Zhao Y, Band M, Chen J. J Cell Biol. 2010 Jun 28;189(7):1157-69.