ICC/IF image of ab85222 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85222, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
IHC-P human hepatocarcinoma reacted with ab85222, which was peroxidase-conjugated to the secondary ab, followed by DAB staining.
Anti-ALS antibody (ab85222) at 1/50 dilution + Jurkat cell line lysate at 35 µg
All lanes : Anti-ALS antibody (ab85222) at 1/1000 dilutionLane 1 : mouse spleen tissueLane 2 : Jurkat cell lineLane 3 : mouse liver tissue lysateLysates/proteins at 35 µg per lane.
Anti-ALS antibody (ab85222) at 1/50 dilution + mouse spleen tissue lysate at 35 µg
ab85222 flow cytometry analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.