All lanes : Anti-Alx1 antibody [EPR11331] (ab181101) at 1/2000 dilutionLane 1 : MCF7 cell lysateLane 2 : HepG2 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Flow cytometry analysis of 2% paraformaldehyde fixed MCF7 cells labeling Alx1 with ab181101 at 1/230 dilution (red) or a Rabbit monoclonal IgG Isotype control (green), followed by Goat anti rabbit IgG (FITC) at a 1/50 dilution.
Anti-Alx1 antibody [EPR11331] (ab181101) at 1/10000 dilution + HeLa cell lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution