Anti-Angiomotin like 1 antibody
| Name | Anti-Angiomotin like 1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab84049 |
| Prices | $370.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF |
| Species Reactivities | Human, Mouse, Rabbit, Guinea Pig, Bovine, Cat |
| Antigen | Synthetic peptide within residues: LTQEDPQMVY QSARQEPQGQ EHQVDNTVME KQVRSTQPQQ NNEELPTYEE , corresponding to N terminal amino acids 144-193 of Human Angiomotin like 1 (NP_570899) Run BLAST with Run BLAST with |
| Description | Rabbit Polyclonal |
| Gene | AMOTL1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-Angiomotin like 1 antibody (ab84049) at 1 µg/ml + 293T cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution
ICC/IF image of ab84049 stained HeLa cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84049, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.