Anti-Angiopoietin 2 antibody
| Name | Anti-Angiopoietin 2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab8452 |
| Prices | $390.00 |
| Sizes | 200 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF IHC-F WB |
| Species Reactivities | Mouse, Human |
| Antigen | Synthetic peptide: CNNFRKSVDSTGRRQYQVQNGP , corresponding to amino acids 21-40 of Mouse Angiopoietin 2 |
| Description | Rabbit Polyclonal |
| Gene | ANGPT2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Supernatants of mouse angiopoietin-expressing endothelial cells. Soluble Tie 2 was used to precipitate the angiopoietins to reduce background.Lane 1 - mock Lane 2 - mouse angiopoietin-2 (clone 2-9) expressing cells Lane 3 - mouse angiopoietin-1 (clone 1-15) expressing cells Lane 4 - mouse angiopoietin-1 (clone 1-8) expressing cells Lane 5 - wtMarion Scharpfenecker 2002
ICC/IF image of ab8452 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8452, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunohistochemical analysis of murine brain coronal sections, staining Angiopoietin 2 with ab8452 at 1/500 dilution. Sections were taken from control mice (left) or mice subjected to middle cerebral artery occlusion (right).
Product References
Anti-atherogenic effects of statins: Impact on angiopoietin-2 release from - Anti-atherogenic effects of statins: Impact on angiopoietin-2 release from
Hilbert T, Poth J, Frede S, Klaschik S, Hoeft A, Baumgarten G, Knuefermann P. Biochem Pharmacol. 2013 Nov 15;86(10):1452-60.
Angiopoietin 2 mediates the differentiation and migration of neural progenitor - Angiopoietin 2 mediates the differentiation and migration of neural progenitor
Liu XS, Chopp M, Zhang RL, Hozeska-Solgot A, Gregg SC, Buller B, Lu M, Zhang ZG. J Biol Chem. 2009 Aug 21;284(34):22680-9.
Green tea polyphenol EGCG sensitizes human prostate carcinoma LNCaP cells to - Green tea polyphenol EGCG sensitizes human prostate carcinoma LNCaP cells to
Siddiqui IA, Malik A, Adhami VM, Asim M, Hafeez BB, Sarfaraz S, Mukhtar H. Oncogene. 2008 Mar 27;27(14):2055-63. Epub 2007 Nov 12.
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Liu XS, Zhang ZG, Zhang RL, Gregg S, Morris DC, Wang Y, Chopp M. J Cereb Blood Flow Metab. 2007 Mar;27(3):564-74. Epub 2006 Jul 12.
High glucose increases angiopoietin-2 transcription in microvascular endothelial - High glucose increases angiopoietin-2 transcription in microvascular endothelial
Yao D, Taguchi T, Matsumura T, Pestell R, Edelstein D, Giardino I, Suske G, Rabbani N, Thornalley PJ, Sarthy VP, Hammes HP, Brownlee M. J Biol Chem. 2007 Oct 19;282(42):31038-45. Epub 2007 Aug 1.