Anti-Angiostatin antibody
| Name | Anti-Angiostatin antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab2904 |
| Prices | $391.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF IHC-F WB ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide corresponding to Mouse Angiostatin aa 98-116 |
| Description | Rabbit Polyclonal |
| Gene | PLG |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunocytochemistry/Immunofluorescence analysis of Angiostatin (green) showing staining in the cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2904 in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
Immunocytochemistry/Immunofluorescence analysis of Angiostatin (green) showing staining in the cytoplasm of K562 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab2904 in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. Nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
All lanes : Anti-Angiostatin antibody (ab2904) at 1 µg/mlLane 1 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)Lane 2 : Human Plasma Total Protein Lysate Lane 3 : Testis (Human) Tissue Lysate - adult normal tissue (ab30257)Lysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Product References
Src is activated by the nuclear receptor peroxisome proliferator-activated - Src is activated by the nuclear receptor peroxisome proliferator-activated
Montagner A, Delgado MB, Tallichet-Blanc C, Chan JS, Sng MK, Mottaz H, Degueurce G, Lippi Y, Moret C, Baruchet M, Antsiferova M, Werner S, Hohl D, Saati TA, Farmer PJ, Tan NS, Michalik L, Wahli W. EMBO Mol Med. 2014 Jan;6(1):80-98.
Urinary angiostatin--a novel putative marker of renal pathology chronicity in - Urinary angiostatin--a novel putative marker of renal pathology chronicity in
Wu T, Du Y, Han J, Singh S, Xie C, Guo Y, Zhou XJ, Ahn C, Saxena R, Mohan C. Mol Cell Proteomics. 2013 May;12(5):1170-9.
Folic acid mitigates angiotensin-II-induced blood pressure and renal remodeling. - Folic acid mitigates angiotensin-II-induced blood pressure and renal remodeling.
Pushpakumar SB, Kundu S, Metreveli N, Sen U. PLoS One. 2013 Dec 26;8(12):e83813.
Mitochondrial division/mitophagy inhibitor (Mdivi) ameliorates pressure overload - Mitochondrial division/mitophagy inhibitor (Mdivi) ameliorates pressure overload
Givvimani S, Munjal C, Tyagi N, Sen U, Metreveli N, Tyagi SC. PLoS One. 2012;7(3):e32388.
Hydrogen sulfide mitigates cardiac remodeling during myocardial infarction via - Hydrogen sulfide mitigates cardiac remodeling during myocardial infarction via
Qipshidze N, Metreveli N, Mishra PK, Lominadze D, Tyagi SC. Int J Biol Sci. 2012;8(4):430-41.
Pretreatment with the probiotic VSL#3 delays transition from inflammation to - Pretreatment with the probiotic VSL#3 delays transition from inflammation to
Appleyard CB, Cruz ML, Isidro AA, Arthur JC, Jobin C, De Simone C. Am J Physiol Gastrointest Liver Physiol. 2011 Dec;301(6):G1004-13. doi:
Effects of D-4F on vasodilation, oxidative stress, angiostatin, myocardial - Effects of D-4F on vasodilation, oxidative stress, angiostatin, myocardial
Weihrauch D, Xu H, Shi Y, Wang J, Brien J, Jones DW, Kaul S, Komorowski RA, Csuka ME, Oldham KT, Pritchard KA. Am J Physiol Heart Circ Physiol. 2007 Sep;293(3):H1432-41. Epub 2007 May 11.
Altered angiogenic balance in ulcerative colitis: a key to impaired healing? - Altered angiogenic balance in ulcerative colitis: a key to impaired healing?
Sandor Z, Deng XM, Khomenko T, Tarnawski AS, Szabo S. Biochem Biophys Res Commun. 2006 Nov 10;350(1):147-50. Epub 2006 Sep 15.
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Balaggan KS, Binley K, Esapa M, MacLaren RE, Iqball S, Duran Y, Pearson RA, Kan O, Barker SE, Smith AJ, Bainbridge JW, Naylor S, Ali RR. Gene Ther. 2006 Aug;13(15):1153-65. Epub 2006 Jun 13.