Immunohistochemical staining(Formalin/PFA-fixed paraffin-embedded sections) of human smooth muscle with ab122320 at 1/250 dilution showing strong cytoplasmic positivity in smooth muscle cells
All lanes : Anti-ANKRD23 antibody (ab122320) at 1/250 dilutionLane 1 : Negative control (vector only transfected HEK293T lysate) Lane 2 : Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)
Immunofluorescent staining of human cell line A-431 (Fixation/Permeabilization: PFA/Triton X-100) with ab122320 at 4ug/ml shows positivity in nucleus but not nucleoli & cytoplasm.