All lanes : Anti-ANKRD53 antibody [2E4] (ab119189) at 1/500 dilutionLane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNALane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY ANKRD53 cDNALysates/proteins at 5 µg per lane.
ab111899 at 1/100 dilution staining ANKRD53 in HEK293T cells transfected with either ANKRD53 overexpression plasmid (red) or empty vector control plasmid (blue) by flow cytometry.
ab119189 at 1/100 dilution staining ANKRD53 in HeLa cells by Immunofluorescence.
ab119189 at 1/100 dilution staining ANKRD53 in Jurkat cells by Immunofluorescence.