Anti-Annexin V antibody [EPR3980]

• Supplier: Abcam
• Catalog: ab108194
• Prices: $387.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR3980
• Applications: WB FC ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Human
• Antigen: A synthetic peptide corresponding to residues in Human Annexin V
• Description: Rabbit Monoclonal
• Gene: ANXA5
• Conjugate: Unconjugated

Product images

Overlay histogram showing HeLa cells stained with ab108194 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab108194, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

All lanes : Anti-Annexin V antibody [EPR3980] (ab108194)Lane 1 : HeLa cell lysateLane 2 : Fetal kidney lysateLane 3 : Fetal brain lysateLane 4 : Jurkat cell lysateLane 5 : JAR cell lysateLysates/proteins at 10 µg per lane.

Immunofluorescent staining of HeLa cells using 1/500 ab108194.

Immunofluorescent staining of staurosporin-treated apoptotic HeLa cells using 1/250 ab108194.

Product References

An atlas of histone deacetylase expression in breast cancer: fluorescence - An atlas of histone deacetylase expression in breast cancer: fluorescence

Ververis K, Karagiannis TC. Am J Transl Res. 2012;4(1):24-43. Epub 2012 Jan 5.