Anti-Annexin VI antibody [EPR17308] - N-terminal

• Supplier: Abcam
• Catalog: ab199422
• Prices: $347.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR17308
• Applications: ICC/IF ICC/IF WB
• Species Reactivities: Mouse, Rat, Human
• Antigen: Recombinant fragment within Human Annexin VI aa 1-100 (N terminal)
• Description: Rabbit Monoclonal
• Gene: ANXA6
• Conjugate: Unconjugated

Product images

All lanes : Anti-Annexin VI antibody [EPR17308] - N-terminal (ab199422) at 1/10000 dilutionLane 1 : Raji (Human Burkitt's lymphoma) whole cell lysateLane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma ) whole cell lysateLane 3 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilutiondeveloped using the ECL technique

All lanes : Anti-Annexin VI antibody [EPR17308] - N-terminal (ab199422) at 1/1000 dilutionLane 1 : Mouse brain tissue lysateLane 2 : Mouse kidney tissue lysateLane 3 : Mouse spleen tissue lysateLane 4 : Rat brain tissue lysateLane 5 : Rat heart tissue lysateLane 6 : Rat kidney tissue lysateLane 7 : Rat spleen tissue lysateLane 8 : PC-12 (Rat adrenal gland pheochromocytoma) cell lysateLane 9 : NIH/3T3 (Mouse embryo fibroblast) whole cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilutiondeveloped using the ECL technique

Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized K562 (Human chronic myelogenous leukemia cells from bone marrow) cells labeling Annexin VI with ab199422 at 1/100, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 (green). Cytoplasm staining on K562 cell line is observed (Subcellular Location: Cytoplasm [UniProt]). The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 (red).The negative controls are as follows:--ve control 1 - ab199422 at 1/1000 followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500.-ve control 2. - ab7291 (anti-Tubulin mouse mAb) at 1/1000 followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500.

Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling Annexin VI with ab199422 at 1/100, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 (green). Cytoplasm staining on HeLa cell line is observed (Subcellular Location: Cytoplasm [UniProt]). The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 (red).The negative controls are as follows:--ve control 1 - ab199422 at 1/1000 followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500.-ve control 2. - ab7291 (anti-Tubulin mouse mAb) at 1/1000 followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500.

Annexin VI was immunoprecipitated from 1mg of Raji (Human Burkitt's lymphoma cell line) whole cell extract with ab199422 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab199422 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.Lane 1: Raji whole cell extract 10 µg (Input).Lane 2: ab199422 IP in Raji whole cell extract.Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199422 in Raji whole cell extract.Blocking and dilution buffer and concentration: 5% NFDM/TBST.