Anti-Annexin VII antibody [EPR16173] - N-terminal (ab198990) at 1/20000 dilution + U87-MG cell lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Anti-Annexin VII antibody [EPR16173] - N-terminal (ab198990) at 1/20000 dilution + Jurkat cell lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-Annexin VII antibody [EPR16173] - N-terminal (ab198990) at 1/5000 dilutionLane 1 : Human fetal kidney lysateLane 2 : Human spleen lysateLysates/proteins at 10 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
All lanes : Anti-Annexin VII antibody [EPR16173] - N-terminal (ab198990) at 1/5000 dilutionLane 1 : Mouse kidney lysateLane 2 : Mouse spleen lysateLane 3 : Rat kidney lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded human cervix carcinoma tissue sections labeling Annexin VII with ab198990 at a 1/200 dilution. Goat anti-rabbit IgG H&L (HRP) ab97051 used as the secondary at a 1/500 dilution. Counterstain hematoxylin.
Annexin VII was immunoprecipitated from U87-MG (human glioblastoma) whole cell extract with ab198990 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab198990 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.Lane 1: U87-MG (human glioblastoma) whole cell extract (Input) 10 µg. Lane 2: ab198990 IP in U87-MG (human glioblastoma) whole cell extract. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab198990 in U87-MG (human glioblastoma) whole cell extract.Blocking and dilution buffer and concentration: 5% NFDM/TBST.