![All lanes : Anti-Annexin VII antibody [EPR17090] (ab197586) at 1/2000 dilutionLane 1 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysateLane 2 : SH-SY5Y (Human neuroblastoma from bone marrow cells) whole cell lysateLane 3 : Rat brain lysateLane 4 : C6 (Rat glial tumor cells) whole cell lysateLane 5 : Raw264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysateLane 6 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysateLane 7 : NIH/3T3 (Mouse embryo fibroblast cells) whole cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/7606_ab197586-237660-ab197586WB.jpg)
All lanes : Anti-Annexin VII antibody [EPR17090] (ab197586) at 1/2000 dilutionLane 1 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysateLane 2 : SH-SY5Y (Human neuroblastoma from bone marrow cells) whole cell lysateLane 3 : Rat brain lysateLane 4 : C6 (Rat glial tumor cells) whole cell lysateLane 5 : Raw264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysateLane 6 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysateLane 7 : NIH/3T3 (Mouse embryo fibroblast cells) whole cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
![Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling Annexin VII with ab197586 at 1/600 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500. Cytoplasm staining on Human cervix carcinoma tissue is observed. Subcellular location citied as Cytoplasm [J Cell Sci. 1995 Jan;108 ( Pt 1):85-95.]. Counter stained with Hematoxylin.Negative control: Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/7607_ab197586-237664-ab197586IHC1.jpg)
Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling Annexin VII with ab197586 at 1/600 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500. Cytoplasm staining on Human cervix carcinoma tissue is observed. Subcellular location citied as Cytoplasm [J Cell Sci. 1995 Jan;108 ( Pt 1):85-95.]. Counter stained with Hematoxylin.Negative control: Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500.
![Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Annexin VII with ab197586 at 1/600 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500. Cytoplasm staining on Mouse liver tissue is observed. Subcellular location citied as Cytoplasm [J Cell Sci. 1995 Jan;108 ( Pt 1):85-95.]. Counter stained with Hematoxylin.Negative control: Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/7608_ab197586-237665-ab197586IHC2.jpg)
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Annexin VII with ab197586 at 1/600 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500. Cytoplasm staining on Mouse liver tissue is observed. Subcellular location citied as Cytoplasm [J Cell Sci. 1995 Jan;108 ( Pt 1):85-95.]. Counter stained with Hematoxylin.Negative control: Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500.
![Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SH-SY5Y (Human neuroblastoma from bone marrow cells) cells labeling Annexin VII with ab197586 at 1/100, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 (green). Cytoplasm staining on SH-SY5Y cell line is observed. Subcellular location is citied as Cytoplasm [J Cell Sci. 1995 Jan;108 ( Pt 1):85-95.] The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 (red).The negative controls are as follows:--ve control 1 - ab179586 at 1/100 followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500.-ve control 2. - ab7291 (anti-Tubulin mouse mAb) at 1/1000 followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/7609_ab197586-239913-ab197586-if-1.jpg)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SH-SY5Y (Human neuroblastoma from bone marrow cells) cells labeling Annexin VII with ab197586 at 1/100, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 (green). Cytoplasm staining on SH-SY5Y cell line is observed. Subcellular location is citied as Cytoplasm [J Cell Sci. 1995 Jan;108 ( Pt 1):85-95.] The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 (red).The negative controls are as follows:--ve control 1 - ab179586 at 1/100 followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500.-ve control 2. - ab7291 (anti-Tubulin mouse mAb) at 1/1000 followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500.
![Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Neuro-2a (Mouse neuroblastoma cells) cells labeling Annexin VII with ab197586 at 1/100, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 (green). Cytoplasm staining on Neuro-2a cell line is observed. Subcellular location is citied as Cytoplasm [J Cell Sci. 1995 Jan;108 ( Pt 1):85-95.] The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 (red).The negative controls are as follows:--ve control 1 - ab179586 at 1/100 followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500.-ve control 2. - ab7291 (anti-Tubulin mouse mAb) at 1/1000 followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/7610_ab197586-237666-ab197586IF1.jpg)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Neuro-2a (Mouse neuroblastoma cells) cells labeling Annexin VII with ab197586 at 1/100, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 (green). Cytoplasm staining on Neuro-2a cell line is observed. Subcellular location is citied as Cytoplasm [J Cell Sci. 1995 Jan;108 ( Pt 1):85-95.] The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 (red).The negative controls are as follows:--ve control 1 - ab179586 at 1/100 followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500.-ve control 2. - ab7291 (anti-Tubulin mouse mAb) at 1/1000 followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500.
Flow cytometric analysis of 2% paraformaldehyde-fixed SH-SY5Y (Human neuroblastoma from bone marrow) cells labeling Annexin VII with ab197586 at 1/60 (red) compared with a rabbit monoclonal IgG isotype control (ab172730) (black) and a unlabelled control (cells without incubation with primary antibody and secondary antibody (blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.