All lanes : Anti-ANP32C antibody [EPR13354] (ab181242) at 1/10000 dilutionLane 1 : HUVEC cell lysateLane 2 : PC-3 cell lysateLane 3 : BxPC-3 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution
Immunofluorescent analysis of 4% paraformaldehyde-fixed PC-3 cells labeling ANP32C with ab181242 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor®555) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).
Flow cytometric analysis of 2% paraformaldehyde-fixed MCF-7 cells labeling ANP32C with ab181242 at 1/20 dilution (red), compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
Western blot analysis of ANP32C in MCF-7 cell lysate immunoprecipitated with ab181242 at 1/50 dilution.Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.