All lanes : Anti-ANP32C antibody [EPR13354-70R] (ab189921) at 1/10000 dilutionLane 1 : MCF-7 cell lysateLane 2 : PC-3 cell lysateLane 3 : BxPC-3 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
All lanes : Anti-ANP32C antibody [EPR13354-70R] (ab189921) at 1/2000 dilutionLane 1 : C6 cell lysateLane 2 : Raw264.7 cell lysateLane 3 : NIH/3T3 cell lysateLysates/proteins at 10 per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Western blot analysis of ANP32C in MCF-7 cell lysate immunoprecipitated using ab189921 at 1/50 dilution (Lane1). Lane 2: PBS instead of MCF-7 lysate.Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.