Anti-APC antibody
| Name | Anti-APC antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab15270 |
| Prices | $392.00 |
| Sizes | 500 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB IP IHC-P |
| Species Reactivities | Mouse, Human, Xenopus, Rat |
| Antigen | Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (Human)(C terminal) |
| Description | Rabbit Polyclonal |
| Gene | APC |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab15270 stained A431 cells. The cells were 4% paraformalehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15270, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Normal human colon section stained with ab15270 (1:100 for 10 min at RT). Sections were pre-treated by boiling in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Product References
Oncogenic transformation of diverse gastrointestinal tissues in primary organoid - Oncogenic transformation of diverse gastrointestinal tissues in primary organoid
Li X, Nadauld L, Ootani A, Corney DC, Pai RK, Gevaert O, Cantrell MA, Rack PG, Neal JT, Chan CW, Yeung T, Gong X, Yuan J, Wilhelmy J, Robine S, Attardi LD, Plevritis SK, Hung KE, Chen CZ, Ji HP, Kuo CJ. Nat Med. 2014 Jul;20(7):769-77.
A genomic toolkit to investigate kinesin and myosin motor function in cells. - A genomic toolkit to investigate kinesin and myosin motor function in cells.
Maliga Z, Junqueira M, Toyoda Y, Ettinger A, Mora-Bermudez F, Klemm RW, Vasilj A, Guhr E, Ibarlucea-Benitez I, Poser I, Bonifacio E, Huttner WB, Shevchenko A, Hyman AA. Nat Cell Biol. 2013 Mar;15(3):325-34.
A relay mechanism between EB1 and APC facilitate STIM1 puncta assembly at - A relay mechanism between EB1 and APC facilitate STIM1 puncta assembly at
Asanov A, Sherry R, Sampieri A, Vaca L. Cell Calcium. 2013 Sep;54(3):246-56.
Evidence that descending cortical axons are essential for thalamocortical axons - Evidence that descending cortical axons are essential for thalamocortical axons
Chen Y, Magnani D, Theil T, Pratt T, Price DJ. PLoS One. 2012;7(3):e33105.
Apc bridges Wnt/beta-catenin and BMP signaling during osteoblast differentiation - Apc bridges Wnt/beta-catenin and BMP signaling during osteoblast differentiation
Miclea RL, van der Horst G, Robanus-Maandag EC, Lowik CW, Oostdijk W, Wit JM, Karperien M. Exp Cell Res. 2011 Jun 10;317(10):1411-21.
Adenomatous polyposis coli is required for early events in the normal growth and - Adenomatous polyposis coli is required for early events in the normal growth and
Ivaniutsin U, Chen Y, Mason JO, Price DJ, Pratt T. Neural Dev. 2009 Jan 16;4:3.