Anti-APE1 antibody [13B8E5C2] - ChIP Grade

• Supplier: Abcam
• Catalog: ab194
• Prices: $395.00
• Sizes: 50 µl
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone: 13B8E5C2
• Applications: ICC/IF ICC/IF IHC-F IP WB ELISA ICC/IF IHC-P ChIP
• Species Reactivities: Mouse, Rat, Human
• Antigen: Full length native protein (purified) (Human)
• Description: Mouse Monoclonal
• Gene: APEX1
• Conjugate: Unconjugated

Product images

ab194 staining cultured human HeLa cells by ICC/IF.  Cells were PFA fixed and permeabilized in 0.5% Triton X100 prior to blocking in 5% BSA for 1 hour at 20°C.  The primary antibody was diluted 1/200 and incubated with the sample for 1 hour at 20°C.  A Cy3® conjugated donkey anti-mouse antibody diluted was used as the secondary.See Abreview

Ab194 staining Human normal placenta. Staining is localized to the nucleus.Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Product References

Identification of novel DNA-damage tolerance genes reveals regulation of - Identification of novel DNA-damage tolerance genes reveals regulation of

Ziv O, Zeisel A, Mirlas-Neisberg N, Swain U, Nevo R, Ben-Chetrit N, Martelli MP, Rossi R, Schiesser S, Canman CE, Carell T, Geacintov NE, Falini B, Domany E, Livneh Z. Nat Commun. 2014 Nov 25;5:5437.

17beta-estradiol increases expression of the oxidative stress response and DNA - 17beta-estradiol increases expression of the oxidative stress response and DNA

Dietrich AK, Humphreys GI, Nardulli AM. J Steroid Biochem Mol Biol. 2013 Nov;138:410-20. doi:

Genomic instability in liver cells caused by an LPS-induced bystander-like - Genomic instability in liver cells caused by an LPS-induced bystander-like

Kovalchuk I, Walz P, Thomas J, Kovalchuk O. PLoS One. 2013 Jul 9;8(7):e67342.

Coordination of MYH DNA glycosylase and APE1 endonuclease activities via physical - Coordination of MYH DNA glycosylase and APE1 endonuclease activities via physical

Luncsford PJ, Manvilla BA, Patterson DN, Malik SS, Jin J, Hwang BJ, Gunther R, Kalvakolanu S, Lipinski LJ, Yuan W, Lu W, Drohat AC, Lu AL, Toth EA. DNA Repair (Amst). 2013 Dec;12(12):1043-52.

Ubiquitin ligase UBR3 regulates cellular levels of the essential DNA repair - Ubiquitin ligase UBR3 regulates cellular levels of the essential DNA repair

Meisenberg C, Tait PS, Dianova II, Wright K, Edelmann MJ, Ternette N, Tasaki T, Kessler BM, Parsons JL, Kwon YT, Dianov GL. Nucleic Acids Res. 2012 Jan;40(2):701-11.

DNA mismatch repair proteins are required for efficient herpes simplex virus 1 - DNA mismatch repair proteins are required for efficient herpes simplex virus 1

Mohni KN, Mastrocola AS, Bai P, Weller SK, Heinen CD. J Virol. 2011 Dec;85(23):12241-53.

Aging results in differential regulation of DNA repair pathways in pachytene - Aging results in differential regulation of DNA repair pathways in pachytene

Paul C, Nagano M, Robaire B. Biol Reprod. 2011 Dec;85(6):1269-78.

Pituitary adenylate cyclase-activating polypeptide prevents cisplatin-induced - Pituitary adenylate cyclase-activating polypeptide prevents cisplatin-induced

Li M, Balamuthusamy S, Khan AM, Maderdrut JL, Simon EE, Batuman V. J Mol Neurosci. 2011 Jan;43(1):58-66.

Direct interaction between XRCC1 and UNG2 facilitates rapid repair of uracil in - Direct interaction between XRCC1 and UNG2 facilitates rapid repair of uracil in

Akbari M, Solvang-Garten K, Hanssen-Bauer A, Lieske NV, Pettersen HS, Pettersen GK, Wilson DM 3rd, Krokan HE, Otterlei M. DNA Repair (Amst). 2010 Jul 1;9(7):785-95.

The carotenoid beta-cryptoxanthin stimulates the repair of DNA oxidation damage - The carotenoid beta-cryptoxanthin stimulates the repair of DNA oxidation damage

Lorenzo Y, Azqueta A, Luna L, Bonilla F, Dominguez G, Collins AR. Carcinogenesis. 2009 Feb;30(2):308-14.