Anti-Apg12 antibody [EPR4799] - Autophagosome Marker

• Supplier: Abcam
• Catalog: ab109492
• Prices: $385.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR4799
• Applications: ICC/IF WB IP IHC-P FC
• Species Reactivities: Human
• Antigen: A synthetic peptide corresponding to residues in Human Apg12
• Description: Rabbit Monoclonal
• Gene: ATG12
• Conjugate: Unconjugated

Product images

Overlay histogram showing HepG2 cells stained with ab109492 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109492, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

All lanes : Anti-Apg12 antibody [EPR4799] - Autophagosome Marker (ab109492) at 1/1000 dilutionLane 1 : THP1 lysateLane 2 : HepG2 lysateLane 3 : Raji lysateLane 4 : Human fetal kidney lysateLysates/proteins at 10 µg per lane.

All lanes : Anti-Apg12 antibody [EPR4799] - Autophagosome Marker (ab109492) at 1/1000 dilutionLane 1 : Apg12-transfected 293T lysateLane 2 : Non-transfected 293T lysateLysates/proteins at 10 µg per lane.

ab109492 at 1/50 dilution staining Apg12 in paraffin embedded Human colon tissue.