Anti-ApoER2 antibody
| Name | Anti-ApoER2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab58216 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Applications | WB ICC/IF ICC/IF FC |
| Species Reactivities | Rat, Human |
| Antigen | Recombinant fragment, corresponding to amino acids 83-171 of Human ApoER2 |
| Description | Mouse Monoclonal |
| Gene | LRP8 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Western blot against tagged recombinant protein immunogen using ab58216 ApoER2 antibody at 1ug/ml.
![Overlay histogram showing PC12 cells stained with ab58216 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab58216, 2µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in PC12 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.Please note that Abcam does not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/8366_ApoER2-Primary-antibodies-ab58216-1.jpg)
Overlay histogram showing PC12 cells stained with ab58216 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab58216, 2µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in PC12 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.Please note that Abcam does not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.
Product References
A novel pathway for human endothelial cell activation by - A novel pathway for human endothelial cell activation by
Allen KL, Fonseca FV, Betapudi V, Willard B, Zhang J, McCrae KR. Blood. 2012 Jan 19;119(3):884-93.
Activated protein C ligation of ApoER2 (LRP8) causes Dab1-dependent signaling in - Activated protein C ligation of ApoER2 (LRP8) causes Dab1-dependent signaling in
Yang XV, Banerjee Y, Fernandez JA, Deguchi H, Xu X, Mosnier LO, Urbanus RT, de Groot PG, White-Adams TC, McCarty OJ, Griffin JH. Proc Natl Acad Sci U S A. 2009 Jan 6;106(1):274-9.