Anti-Apolipoprotein A I antibody

• Supplier: Abcam
• Catalog: ab20453
• Prices: $384.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF RID WB ELISA IHC-F ELISA
• Species Reactivities: Mouse, Rat
• Antigen: Purified mouse apolipoprotein AI from pooled mouse plasma high density lipoprotein
• Description: Rabbit Polyclonal
• Gene: Apoa1
• Conjugate: Unconjugated

Product images

ab20453 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab20453 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

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