Anti-Apolipoprotein B antibody
| Name | Anti-Apolipoprotein B antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab7616 |
| Prices | $384.00 |
| Sizes | 100 µg |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | DB ELISA IHC-P WB ICC/IF ICC/IF |
| Species Reactivities | Rabbit, Bovine, Human |
| Antigen | Full length native apoLipoprotein Type B (purified) |
| Description | Goat Polyclonal |
| Gene | APOB |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-Apolipoprotein B antibody (ab7616) at 1 µg/ml + Human Apolipoprotein B full length protein (ab77859) at 0.01 µgSecondaryRabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilutiondeveloped using the ECL techniqueExposure time : 3 minutes
ab7616 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab7616 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 donkey anti- goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Effects of nonesterified fatty acids on the synthesis and assembly of very low - Effects of nonesterified fatty acids on the synthesis and assembly of very low
Liu L, Li X, Li Y, Guan Y, Song Y, Yin L, Chen H, Lei L, Liu J, Li X, Wang Z, Yang X, Liu G. J Dairy Sci. 2014 Mar;97(3):1328-35.
High-density lipoprotein impedes glycation of low-density lipoprotein. - High-density lipoprotein impedes glycation of low-density lipoprotein.
Younis NN, Soran H, Charlton-Menys V, Sharma R, Hama S, Pemberton P, Elseweidy MM, Durrington PN. Diab Vasc Dis Res. 2013 Mar;10(2):152-60.
Intraretinal leakage and oxidation of LDL in diabetic retinopathy. - Intraretinal leakage and oxidation of LDL in diabetic retinopathy.
Wu M, Chen Y, Wilson K, Chirindel A, Ihnat MA, Yu Y, Boulton ME, Szweda LI, Ma JX, Lyons TJ. Invest Ophthalmol Vis Sci. 2008 Jun;49(6):2679-85.
Role of inflammation in nonrheumatic, regurgitant heart valve disease. A - Role of inflammation in nonrheumatic, regurgitant heart valve disease. A
Wallby L, Steffensen T, Broqvist M. Cardiovasc Pathol. 2007 May-Jun;16(3):171-8.
Lipoprotein inflammatory properties and serum amyloid A levels but not - Lipoprotein inflammatory properties and serum amyloid A levels but not
Van Lenten BJ, Wagner AC, Navab M, Anantharamaiah GM, Hama S, Reddy ST, Fogelman AM. J Lipid Res. 2007 Nov;48(11):2344-53. Epub 2007 Aug 10.
Increased apolipoprotein deposits in early atherosclerotic lesions distinguish - Increased apolipoprotein deposits in early atherosclerotic lesions distinguish
Wyler von Ballmoos M, Dubler D, Mirlacher M, Cathomas G, Muser J, Biedermann BC. Arterioscler Thromb Vasc Biol. 2006 Feb;26(2):359-64. Epub 2005 Dec 1.
Atherin: a newly identified, lesion-specific, LDL-binding protein in human - Atherin: a newly identified, lesion-specific, LDL-binding protein in human
Lees AM, Deconinck AE, Campbell BD, Lees RS. Atherosclerosis. 2005 Oct;182(2):219-30.