![Anti-Apolipoprotein D antibody [EPR2916] (ab108191) at 1/1000 dilution + Human plasma cell lysate at 10 µg](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/8501_Apolipoprotein-D-Primary-antibodies-ab108191-1.jpg)
Anti-Apolipoprotein D antibody [EPR2916] (ab108191) at 1/1000 dilution + Human plasma cell lysate at 10 µg
Immunohistochemical staining of Apolipoprotein D in paraffin embedded Human kidney tissue, using ab108191 at a 1/100 dilution.
Immunohistochemical staining of Apolipoprotein D in paraffin embedded Human liver tissue, using ab108191 at a 1/100 dilution.
ICC/IF image of ab108191 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab108191, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.