Anti-Apolipoprotein E antibody
| Name | Anti-Apolipoprotein E antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab7620 |
| Prices | $391.00 |
| Sizes | 100 µg |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ELISA WB ICC/IF ICC/IF |
| Species Reactivities | Rat, Human |
| Antigen | Full length native protein (purified)(Human) (isolated from human plasma by density gradient centrifugation followed by HPLC purification) |
| Description | Goat Polyclonal |
| Gene | APOE |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-Apolipoprotein E antibody (ab7620) at 1/1000 dilution (in PBS/Tween (0.01%) + 3% milk for 12 hours) + Whole cell lysate of Rat glial cells at 40 µgSecondaryAn HRP-conjugated Rabbit anti-goat Polyclonal at 1/200 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ICC/IF image of ab7620 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab7620 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 donkey anti- goat (ab96931) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Aberrant glycosylation of plasma proteins in severe preeclampsia promotes - Aberrant glycosylation of plasma proteins in severe preeclampsia promotes
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Novel role of ADAMTS-5 protein in proteoglycan turnover and lipoprotein retention - Novel role of ADAMTS-5 protein in proteoglycan turnover and lipoprotein retention
Didangelos A, Mayr U, Monaco C, Mayr M. J Biol Chem. 2012 Jun 1;287(23):19341-5.
Dramatic caspase-dependent apoptosis in antibody-enhanced dengue virus infection - Dramatic caspase-dependent apoptosis in antibody-enhanced dengue virus infection
Brown MG, Huang YY, Marshall JS, King CA, Hoskin DW, Anderson R. J Leukoc Biol. 2009 Jan;85(1):71-80.
An altered pattern of circulating apolipoprotein E3 isoforms is implicated in - An altered pattern of circulating apolipoprotein E3 isoforms is implicated in
Atkinson KR, Blumenstein M, Black MA, Wu SH, Kasabov N, Taylor RS, Cooper GJ, North RA. J Lipid Res. 2009 Jan;50(1):71-80.